American Society of Microbiology ABMM

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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is investigating the development of a broadly protective vaccine against coronaviruses.
They are focusing on conserved epitopes in the spike (S) protein that are accessible to neutralizing antibodies across different beta-coronaviruses. Which region of the S protein is considered a promising target for such a vaccine?

A) The S1 subunit, which is primarily responsible for host cell receptor binding.
B) The N-terminal domain (NTD) of the S protein.
C) The S2 subunit, which is more conserved and mediates membrane fusion.
D) The receptor-binding domain (RBD), which exhibits high sequence variability.

2. Histopathological examination of lung tissue from an immunocompromised patient reveals large (8-15 µm), spherical yeast cells with thick, refractile walls and characteristic single, broad-based budding. These findings are most suggestive of infection with:

A) Blastomyces dermatitidis
B) Histoplasma capsulatum
C) Coccidioides immitis/posadasii
D) Paracoccidioides brasiliensis

3. A research team is investigating the mechanism of bacterial resistance to a novel fluoroquinolone antibiotic.
They isolate a resistant strain of Escherichia coli from a patient with a urinary tract infection. Genetic analysis of this strain reveals a point mutation in the gyrA gene. This mutation MOST likely results in:

A) Modification of the ribosomal target site, preventing antibiotic binding.
B) Alteration of the DNA gyrase enzyme, reducing its affinity for the fluoroquinolone.
C) Production of an enzyme that inactivates the fluoroquinolone molecule.
D) Increased expression of efflux pumps that actively transport the antibiotic out of the cell.

4. A patient with a history of liver transplantation develops a bloodstream infection with a Gram-negative bacterium. The isolate is identified as Sphingomonas paucimobilis. This opportunistic pathogen is known for its ability to:

A) Produce potent exotoxins that directly damage host tissues.
B) Degrade a wide range of environmental pollutants.
C) Exhibit intrinsic resistance to a broad spectrum of antibiotics.
D) Form biofilms on indwelling catheters and prosthetic devices.

5. Transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease (CJD) in humans and Bovine Spongiform Encephalopathy (BSE) in cattle, are caused by infectious agents composed primarily of:

A) Aberrantly folded host-encoded prion proteins (PrP<sup>Sc</sup>)
B) Viroids (small, circular RNA molecules)
C) Defective viral particles requiring a helper virus
D) Slow-growing bacteria lacking a cell wall

Solutions: